Adding vinegar or lemon juice too early in the mayonnaise process can prevent the emulsion from forming properly, resulting in a thin, separated mayonnaise. The acid interferes with the initial binding of the egg yolk and oil.
Mayonnaise is an emulsion, a stable mixture of oil and water-based liquids (in this case, egg yolk and vinegar/lemon juice) that normally wouldn't mix. The egg yolk acts as an emulsifier, containing lecithin, which helps bind the oil and water together.
The process requires a gradual addition of oil to the egg yolk, allowing the lecithin to properly coat the oil droplets and suspend them in the water-based liquid. Introducing the vinegar or lemon juice too early disrupts this process in a couple of ways:
Acid Interference: The acidity can denature the proteins in the egg yolk, hindering their ability to emulsify the oil effectively.
Premature Thinning: Adding the acid too early thins the mixture before a stable emulsion has formed. This makes it difficult for the oil to properly incorporate, leading to separation.
The correct method is to start with the egg yolk and a small amount of mustard (optional, but it helps stabilize the emulsion), then slowly drizzle in the oil while whisking vigorously. Once a stable emulsion has formed and the mayonnaise has thickened, you can then add the vinegar or lemon juice to taste.
If your mayonnaise separates, don't throw it out! Try whisking another egg yolk in a clean bowl and then slowly drizzling the separated mayonnaise into the new yolk, whisking constantly. This can often re-emulsify the mixture and save your mayonnaise.