Over-kneading white bread dough results in a tough, dense bread with a crumbly texture due to the gluten strands becoming too tight and breaking down.
Kneading develops the gluten in wheat flour, creating a network of protein strands that give bread its structure and elasticity. When dough is kneaded properly, these strands align and become strong, allowing the dough to trap gas produced by yeast, resulting in a light and airy loaf. However, over-kneading stretches and tightens these gluten strands excessively. Eventually, the strands become so tight that they begin to break down. This breakdown weakens the dough's structure, preventing it from holding its shape during baking. The resulting bread will be dense, tough, and may have a crumbly or uneven texture. The dough may also become slack and sticky, making it difficult to handle.
To avoid over-kneading, use the windowpane test. After kneading for a few minutes, stretch a small piece of dough between your fingers. If you can stretch it thin enough to see light through it without tearing, the gluten is sufficiently developed. If it tears easily, continue kneading for a short time and test again.