Acid prevents browning in fruits and vegetables by inhibiting the enzyme polyphenol oxidase (PPO), which requires a specific pH to function, and by consuming the oxygen needed for the browning reaction.
Browning, also known as enzymatic browning, occurs when fruits and vegetables are cut or bruised, exposing their internal tissues to oxygen. This triggers the enzyme polyphenol oxidase (PPO) to react with phenolic compounds present in the plant tissue. This reaction leads to the production of melanins, which are brown pigments.
Acids, such as lemon juice (citric acid) or vinegar (acetic acid), prevent browning through two primary mechanisms:
Lowering pH: PPO enzymes are most active within a specific pH range. By adding acid, the pH is lowered, creating an environment where the enzyme cannot function effectively. This slows down or completely stops the browning reaction.
Reducing Oxygen Availability: Some acids, like ascorbic acid (Vitamin C), act as antioxidants. They react with oxygen before PPO can, effectively consuming the oxygen needed for the browning reaction to occur. This is why Vitamin C is often used to prevent browning in fruit salads.
Therefore, adding an acidic solution to cut fruits and vegetables significantly reduces or eliminates enzymatic browning, preserving their color and appearance.
When using lemon juice to prevent browning, dilute it slightly with water to avoid altering the flavor of the fruit or vegetable too much. A ratio of 1 part lemon juice to 3 parts water is often effective.